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The enzyme does not attack the alpha-carboxyl bond of glutamate in peptides or proteins [11,12]
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To confirm the interaction between Ssu72 and GSK3β more clearly, we conducted an in vitro binding assay using GST or GST-fused GSK3β (GST-GSK3β) incubated with purified His-tagged Ssu72 (His-Ssu72) (Supplementary Fig
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