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Quantitative evaluation of apoptosis was performed by flow cytometry after double staining using fluorescein FITC-conjugated Annexin V and 5 μg/ml Propidium iodide 10 min at room temperature, and analyzed by fluorescence-activated cell sorting (FACS) in the FL1 and FL2 channels to determine the percentage of dead cells

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Nevertheless, it has also been found that H 2 S may have anti-tumor properties by inducing apoptosis and inhibiting cell proliferation