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The sections were stained with a Congo red dye solution (0.5% Congo red in 50% ethanol) for 5 min, differentiated in a 0.2% KOH solution in 80% ethanol for 1 min, washed thrice with deionized water for 5 min, and embedded into the Immu-MountTM aqueous-based mountant (Thermo Scientific)

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Glutathione-S-Transferases (GST) GSTs belong to the Phase II biotransformation enzymes catalyzing the GSH-mediated peroxide reduction [194] and conjugation of GSH with a variety of reactive electrophiles, most commonly generated by cytochrome P450 metabolism [195]