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One set of lysates were mixed with non-reducing 4× loading dye (0.5% TAE buffer, 20% glycerol, 8% Sarkosyl, 0.1 mg/ml bromophenol blue, 1× protease inhibitors, 1× phosphatase inhibitors added) to preserve disulfide bonds
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[15] [18] For borderline results, additional tests such as methylmalonic acid, homocysteine, or holotranscobalamin may be considered
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