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Interestingly, similar depletion kinetics in this experiment could also be observed by substituting (CYS) 2 with oxidized glutathione (GSSG) (Figure 5—figure supplement 1C,D), although intracellular and extracellular levels of GSSG in cell culture were much lower than (CYS) 2 (Figure 5—figure supplement 1E,F), suggesting it is not the major target of SO 3 2-