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The cumulative release was calculated using the following equation: Mt(n) = Vr × Cn + Vs × ƩCm, where Mt(n) is the current cumulative mass of released Dox at time t, n is the number (times) of sampling, Cn is the current concentration of Dox in the medium, ƩCm is the summed total of the previously measured concentrations, Vr is the volume of the medium, and Vs corresponds to the volume of the sample removed for analysis 53

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PubMed, Higashino, Toshihide, et al

Isolation from yeast