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Furthermore, the interactions between these genetic factors and environmental influences add to another layer of complexity

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Viral infection For lentiviral production, HEK293T cells were co-transfected with 4 μg Δ8.91, 1 μg pVSVG, 5 μg of lentivector (shRNA targeting SHMT2 : CCAGAATTTGTAGCTGAGATT, and ATF4 : GCGAGTGTAAGGAGCTAGAAA, obtained from RNA Technology platform and Gene manipulating core, Academia Sinica) by using 30 μL of Turbofect (Thermo, R0533) in 1 mL of Opti-MEM (Gibco, 31985070) according to the manufacturer’s instructions