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On the other hand, with ligation of the inferior caval vein occlusion we made a recapitulation of Virchow

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A 25 µl of mouse plasma was transferred into 1.5 ml centrifuge tube which was spiked with 2.5 µl of internal standard (IS) working standard solution (100 µg/ml) to get final IS contraction of 250 ng/ml and the solution was vortexed