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Briefly, sections of lung tissue were washed with PBS and incubated in fleshly prepared Perls’ solution (5% potassium ferrocyanide (Sigma-Aldrich)/10% hydrochloric acid) for 1 h, followed by a 15 min incubation in DAB

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Proline is converted to hydroxyproline by prolyl hydroxylase, while lysine is converted to hydroxylysine by lysyl hydroxylase