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JAMA (2005) 293(5):557–64

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Size:60 tablets for 1 month

Samples were centrifuged at 20,000 × g , 4 °C for 30 min, the supernatant transferred to a new tube, centrifuged again at 20,000 × g , 4 °C for 10 min, and the final supernatant transferred to a new 1.5 ml microtube