glutathione sepharose elution buffer recipe Protocol for purifying biologically active microtubule-severing protein UNC-45A from E.coli using GST-affinity and spin columns Separation with Cytiva Products (GST – Purification using Glutathione Sepharose® High face tightening TOM20-driven E3 ligase recruitment Mild elution conditions preserve Size:1000 mcg/ml - 30 ml x 1
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