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The solvent was removed under reduced pressure, and the crude product was purified by alumina basic column chromatography (ISCO, 8 g) using MeOH (3–5%) in DCM to get Boc-mitragynine N -oxide ( Boc-MNO ) as a white solid, (yield: 90 mg, 79%)

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Subcutaneous injection means inserting a thin needle into the fat layer beneath your skin at a 45-degree angle