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pET-Ube3C-HECT WT, pET-Ube3C-HECT C1051S, pET-Ube3C-HECT S845F, pET-Ube3C-HECT F996C The fusion of GST-HIS-Ube3C-HECT fragment was created by amplification from a plasmid template (pCAG-myc-Ube3C or respective mutants) using oligos: 5’-aagtcctctttcagggacccGTTCCATTTGAGGAGCGAG-3’ with 5’-gaattcggatcctggtacccttattaGCTCAGCTCAAAGCCAGC-3’ and inserted into the pET-49B+ expression vector (Novagen #71463) using SmaI restriction and NEBuilder system
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