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Thus, experiments evaluating responses to estrogen, progesterone, or testosterone require careful control of serum content

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A prototypical example involves LPS activation of Kupffer cells (KCs) through TLR4 signaling, which promotes the secretion of pro-inflammatory mediators such as CC-chemokine ligand 2 (CCL2), interleukin (IL)-8, tumor necrosis factor-α (TNF-α), IL-1β, and IL-6 [35, 39]