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The respective peak fractions were collected and subunits were pelleted through a 1 M sucrose cushion in LS Buffer (20 mM HEPES/KOH pH 7.5, 100 mM KOAc, 10 mM MgCl 2 , 1 mM DTT) in a Type 70 Ti rotor at 140,900 x g for 14 h