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Bacterial cells were collected by centrifugation at 4,000 g for 20 min at 4 °C and the cell pellet was resuspended in buffer (50 mM NaH 2 PO 4 and 300 mM NaCl, pH 6.0)

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Class I includes HDAC1, 2, 3, and 8, which are usually localized in the nucleus, have deacetylase activity, and contain zinc-dependent active sites ( + as a cofactor ( Table 1 ) ( Figure 1 ) ( Table 1 Classification of HDACs based on homology to human yeast homologs and generalization of their cofactor, size, and cellular localization