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Briefly, 0.5–1 g of ground stem tissue from actively growing 1-month-old Brachypodium and Arabidopsis plants was resuspended in 2.7 ml of extraction buffer (100 mM Tris-Cl pH 7.5, 10% glycerol, 1 mM phenylmethylsulfonyl fluoride, and 0.5 mM dithiothreitol) in the presence of 0.1 g of PVPP