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In vitro drug release studies in simulated intestinal fluid (SIF) and gastric fluids (SGF) revealed that over 80% of the two drugs were released within 5 h in SGF, with a sustained release observed up to 24 h in SIF

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Samples were heated at 80 °C for 15 min to release protein-bound ET, then clarified by protein precipitation with 500 µl of cold acetone overnight at −20 °C