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Reactions were performed with 5 ⁇ M histone H3 (histone L-arginine) substrate, 1 ⁇ M radiolabeled S-adenosyl-L-[methyl-3H]methionine (SAM) co-substrate, and recombinant human PRMT3 enzyme

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The severity and duration of deficiency are primary determinants—patients with mild, recently developed deficiency typically respond more rapidly than those with longstanding, severe depletion

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