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(2) Marker profiling, including tetraspanins (CD63, CD81, CD9) and exclusion of non-EV proteins (e.g., GM130, Calnexin) (3) Cargo analysis, such as protein and RNA content via Western blotting, ELISA, or sequencing

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Direct activators, on the other hand, bind directly to AMPK and activate it without the influence of these nucleotides (Tamura et al., 2022)